The low levels of circulating Doublecortin-like kinase 1 Levels in Breast Cancer


N O., E K., D D., G O., Serilmez M., Ho S., ...Daha Fazla

MITTEILUNGEN KLOSTERNEUBURG, sa.Vol. 40 (n. 2, 2025), ss.4-13, 2025 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.61586/b41ec
  • Dergi Adı: MITTEILUNGEN KLOSTERNEUBURG
  • Derginin Tarandığı İndeksler: Scopus, BIOSIS, CAB Abstracts
  • Sayfa Sayıları: ss.4-13
  • İstanbul Üniversitesi Adresli: Evet

Özet

Purpose: Breast cancer is the most prevalent form of cancer among women worldwide, and is a complex disease. The development of biomarkers for early diagnosis is a promising avenue for improving the treatment of breast cancer. Doublecortin-like kinase 1 (DCLK1) is a member of the protein kinase superfamily and doublecortin family. It has been hypothesised that cells expressing DCLK1 in these tumours exhibit stem cell characteristics, suggesting a potential association with tumour initiation and mortality. The objective of this study was to evaluate the serum protein and mRNA levels of the DCLK1 molecule in patients diagnosed with breast cancer. Methods: The present study comprised 62 female patients diagnosed with breast cancer and 30 healthy controls. DCLK1 serum level was determined by ELISA, and mRNA level was determined by quantitative RT-PCR method before any treatment. Results: Serum DCLK1 level was found to be statistically significantly lower in breast cancer patients (5.38 ± 1.86 ng/mL) than in the control group (7.5 ± 1.15 ng/mL) (p = 0.001). Furthermore, we determined that serum DCLK mRNA expression level was significantly lower in breast cancer patients (0.012-0.09, mean rank: 34.48) compared to the control group (0.04-0.09, mean rank: 61.22) (p <0.001, Z: -4.29). Conclusion: DCLK1 may be a useful marker for detection of the clinical behaviour of breast cancer.