The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit


SAINI S., Tuzun E. , CHRISTADOSS P.

JOURNAL OF NEUROIMMUNOLOGY, vol.169, pp.177-179, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 169
  • Publication Date: 2005
  • Doi Number: 10.1016/j.jneuroim.2005.08.013
  • Title of Journal : JOURNAL OF NEUROIMMUNOLOGY
  • Page Numbers: pp.177-179

Abstract

The mRNA isolated front mouse ocular and limb Muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-alpha subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular Muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products. (C) 2005 Elsevier B.V. All rights reserved.