The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit


SAINI S., Tuzun E. , CHRISTADOSS P.

JOURNAL OF NEUROIMMUNOLOGY, cilt.169, ss.177-179, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 169
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.jneuroim.2005.08.013
  • Dergi Adı: JOURNAL OF NEUROIMMUNOLOGY
  • Sayfa Sayıları: ss.177-179

Özet

The mRNA isolated front mouse ocular and limb Muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-alpha subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular Muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products. (C) 2005 Elsevier B.V. All rights reserved.