Soil salinity is a very important abiotic stress condition that affects plant growth and crop yield. Photosynthetic activity, fresh weight, total protein amount decrease due to salinity condition. Brassinosteroids (BR) are a new group of hormones in the steroidal structure which is involved in the plant hormone group. BRs play an important role in various physiological processes. BR shave a curative effect on the plants exposed to environmental stress. In this study, the effect on seedling development was examined by spraying 24-epibrassinolide (eBL), the active form of brassinosteroids, on the seedlings exposed to salt stress. For this purpose, seedlings are divided into three groups such as Hoagland, Hoagland+NaCl, Hoagland+NaCl+eBL. As a result of preliminary experiments, 150 mM NaCl as the salt concentration reducing seedling growth and 10-9 M eBL which promotes growth by reducing this inhibition were determined as the appropriate concentration. This study; biochemical analysis of spinach seedlings exposed to salt stress and applied to eBL showed the curative effect of eBL on salt toxicity.