Akademik Veri Yönetim Sistemi
IMMUNOLOGY, cilt.78, sa.3, ss.436-443, 1993 (SCI-Expanded)
Gammadelta T cells were isolated from human decidua parietalis, decidua basalis and cervix and cloned in the presence of interleukin-2 (IL-2). T-cell receptor (TcR) expression was then analysed and compared with that of a panel of gammadelta T-cell clones from peripheral blood. Only 17/40 (42.5%) clones from decidua parietalis were Vgamma9+/Vdelta2+ as compared to 68/94 (72%) of peripheral blood clones (P<0.005). Conversely, 50% of clones from decidua parietalis but only 15% of clones from peripheral blood were Vdelta1 + (P < 0.00 1). At least seven distinct TcR types were identified among the panel of clones from decidua parietalis and at least six different types were expressed by the panel of 17 clones from cervix. This receptor heterogeneity was not a result of interdonor variation as in all instances where more than one clone was obtained from a single sample, individual clones having between two and five receptor types were identified. However, 23/24 (95.8%) of clones from decidua basalis were Vgamma9+/Vdelta2+. Most clones from decidua parietalis and cervix, whether Vgamma9+/Vdelta2+ or Vdelta1+, were positive for the mucosal lymphocyte marker, HML-1, but expression was often heterogeneous within a single clone. In contrast, almost all gammadelta T-cell clones from peripheral blood were HML-1 -. Thus, unlike the mouse, gammadelta T cells within these human female reproductive tissues have a diverse TcR repertoire which, in decidua parietalis, is distinct from that of peripheral blood.