Separation and some properties of Aloe vera L. leaf pulp lectins


Akev N., Can A.

PHYTOTHERAPY RESEARCH, vol.13, no.6, pp.489-493, 1999 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 6
  • Publication Date: 1999
  • Journal Name: PHYTOTHERAPY RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.489-493
  • Keywords: Aloe, lectin, phytohaemagglutinin, ALPHA-GALACTOSIDASES, BARBADENSIS MILLER, ARBORESCENS MILLER, PURIFICATION, GEL
  • Istanbul University Affiliated: Yes

Abstract

The separation and partial purification of two lectins from the leaf pulp of Aloe vera L. (=barbadensis Miller) is presented. The fraction shelving haemagglutinating activity was precipitated at 50% ammonium sulphate concentration from the crude leaf pulp extract. The precipitate thus obtained, after dialysis, was applied to a hydroxylapatite column. Stepwise elution resulted in two peaks showing haemagglutinating activity eluted with 5 mM (Aloctin I) and 20 mM (Aloctin II) phosphate buffers. Haemagglutinating activity was estimated visually by adding a 4% rabbit erythrocyte suspension to serial two-fold dilutions of the lectins in microtitration plates. None of the 20 sugars tested inhibited haemagglutinating activity of Aloctin I up a concentration of 500 mM. Aloctin II was inhibited by N-acetyl-D-galactosamine at 250 mh concentration. Of 10 metal ions tested, only Al3+ salts were found to activate Aloctin I and II. On the other hand, it was shown that neither lectin possessed any alpha- and beta- galactosidase or alpha- and beta- glucosidase activity. The lectins were of glycoprotein structure containing approximately 5% neutral sugar. The specificity of the lectins towards human and rat erythrocytes was investigated. Copyright (C) 1999 John Wiley & Sons, Ltd.