Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Öztekin, Nevin; Baskan, Selda; Kepekci, S.; Berker, Fatma; Topcu, Guelacti

doi:10.1016/j.jpba.2009.04.012

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Atıf İçin Kopyala

Öztekin N., Baskan S., Kepekci S. E., Berker F. B., Topcu G.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.51, sa.2, ss.439-442, 2010 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 51 Sayı: 2
Basım Tarihi: 2010
Doi Numarası: 10.1016/j.jpba.2009.04.012
Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.439-442
İstanbul Üniversitesi Adresli: Evet

Özet

In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia Species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 mu g/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts. (C) 2009 Elsevier B.V. All rights reserved.