Genome organization changes in GM and non-GM soybean [Glycine max(L.) Merr.] under salinity stress by retro-transposition events

Sahin O., Karlik E., Meric S., ARI Ş., Gozukirmizi N.

GENETIC RESOURCES AND CROP EVOLUTION, cilt.67, sa.6, ss.1551-1566, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10722-020-00928-1
  • Dergi Adı: GENETIC RESOURCES AND CROP EVOLUTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.1551-1566
  • Anahtar Kelimeler: Genetically modified crops, Transposable elements, Salinity stress, Soybean, GM soybean, SIRE1, Bagy2, Nikita, POTATO SOLANUM-TUBEROSUM, TRANSPOSABLE ELEMENTS, LTR-RETROTRANSPOSONS, GENETIC DIVERSITY, DNA METHYLATION, PLANT RETROTRANSPOSONS, MOLECULAR MARKER, BARLEY, EVOLUTION, MECHANISMS
  • İstanbul Üniversitesi Adresli: Evet

Özet

Genetically modified (GM) crops are developed and cultivated worldwide, providing protection against insects and diseases, or tolerance to herbicides. However, despite the advantages that GM crops, they pose many concerns not only to human and animal health but also to the environment. In the present study, we perform inter-retrotransposon amplified polymorphism (IRAP)-PCR to revealSIRE1,Bagy2andNikitatransposition events in soybean [Glycine max(L.) Merr.] and GM soybean under increasing salt stress conditions (0 mM as control, 80 mm, 120 mM, 160 mM and 200 mM NaCl). Salinity dramatically affected soybean and GM soybean in a similar way that stem lengths, root lengths and leaf areas were reduced compared to control. According to IRAP-PCR results, polymorphism ratios ofSIRE1,Bagy2andNikitaretrotransposons among all samples were found to be 22-100%, 0-73% and 37-100%, respectively. These results are the first direct report to demonstrate the relationship between TEs polymorphisms and salinity stress in soybean and GM soybean. TEs, which may explain part of the new phenotypes, play essential roles in genome and gene evolution. Such information can be useful to understanding of genome organization differences derived from TEs between soybean and GM soybean.