Akademik Veri Yönetim Sistemi
INSTRUMENTATION SCIENCE AND TECHNOLOGY, cilt.2023, ss.1-16, 2023 (SCI-Expanded)
Rapid and easy detection of pathogens, especially bacteria, playsan important role in daily life in the face of increasing environ-mental pollution. Nanosensors focus on carbon nanotubes, whichcan bind various molecules to large surfaces and have high elec-tron conductivity. They are widely used in the development ofelectrochemical biosensors. In this study, a new design of electro-chemical biosensors is presented by integrating gold-coated tung-sten wires (GC-TW) and acid-functionalized multi-walled carbonnanotubes (MWCNTs) in a two-step coating process. GC-TWs arecoated with acid-functionalized short MWCNTs. Fourier transformsinfrared spectroscopy (FTIR), transmission electron microscopy(TEM), and scanning electron microscopy (SEM) results show effi-cient immobilization of the electrodes. The performance forEscherichia coli(E. coli) K-12 is evaluated based upon the changein current following antibody-antigen interactions. Bioimpedancemeasurements are performed from 50 Hz to 10 MHz to achieveoptimal coverage of impedance changes. The measurementsshow that the electrode has a detection limit of 0.8 CFU/mL. Theresponse of the electrode saturates when the bacterial concentra-tion is increased to 1.70 x 102CFU/mL.ThebiosensoriscapableofdetectingE. colibetween 0.8 and 1.70102CFU/mL. The datashow that this MWCNT-based biosensor can be developed into ahighly sensitive device forE. coli.