Effects of β-carotene and porphyridium cruentum polysaccharide extract on mitomycin c sensitivity of blood lymphocyte chromosomes of radiology technicians

ŞENOL S. G., Pehlivan M., ÖZDEMİR G., Conk-Dalay M., Kucuk O., Pehlivan S.

Balkan Journal of Medical Genetics, vol.9, no.1-2, pp.63-66, 2006 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 1-2
  • Publication Date: 2006
  • Journal Name: Balkan Journal of Medical Genetics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.63-66
  • Keywords: β-Carotene, (βC), Chromosome aberration(s), Lymphocyte, Mitomycin C (Mit-C), Porphyridium cruentum (PC), X-rays
  • Istanbul University Affiliated: No


We have investigated the individual and combined effects of β-carotene (βC) and a crude polysaccharide extract of the red alga Porphyridium cruentum (PC) on Mitomycin C (Mit-C) sensitivity of peripheral blood lymphocytes of humans occupationally exposed to low dose gamma radiation. Peripheral blood lymphocytes in vitro were treated with Mit-C and the chromosome abnormalities in the presence or absence of βC and/or PC were recorded. The incidence of chromosomal abnormalities increased from 1.9% in cell cultures without Mit-C to 7.8% in those with Mit-C with a significant increase in chromosomal aberrations (chromatid breaks, chromosome breaks, dicentrics and rings) (p <0.05). However, in Mit-C treated cultures that contained βC+PC the incidence was 1.3%. Mitomycin C-induced aberrations in the lymphocytes decreased significantly with the addition of βC and PC to the culture. This suggests that mutagen sensitivity may be reduced by a βC+PC combination and that this combination may protect humans exposed to radiation.