Association of CYP1A1 and GSTM1 null polymorphisms with pro- and anti- inflammatory cytokines in the OCD group


Karaaslan Z., Küçükali C. İ., Yüceer H., Delen E., Özkök E.

2nd International Molecular Biosciences PhD and Postdoc Conference, Cologne, Germany, 23 - 25 November 2023, pp.71

  • Publication Type: Conference Paper / Summary Text
  • City: Cologne
  • Country: Germany
  • Page Numbers: pp.71
  • Istanbul University Affiliated: Yes

Abstract

It is thought that the activation of the kynurenine pathway causes inflammation and

plays an important role in obsessive-compulsive disorder (OCD) pathogenesis.

Cytochrome P450 (CYP) enzyme activity has been shown to be modified by

inflammatory cytokines. The members of μ of GSTs of the GSTs multigenic family show

a common polymorphism that is characterized by the complete deletion of the gene. It

is known that homozygous deletions (null genotype) result in the absence of catalytic

activity of the GST enzyme. The null genotypes of GSTs have been associated with

oxidative stress-related diseases such as cardiovascular, epilepsy, and psychiatric

diseases. We aimed to examine the relationship between pro-inflammatory cytokine

interleukin-1beta (IL-1β), anti-inflammatory interleukin-10 (IL-10) and interleukin-4 (IL-4),

serum cytochrome P450 (CYP1A1) and glutathione S-transferase1 (GSTM1) null

genotype in OCD. A hundred people with OCD and fifty healthy controls were included in

our study. Enzyme-linked Immunosorbent Assay (ELISA) was used to determine serum

IL-1β, IL-4 and CYP1A1 levels. GSTM1 present/null genotyping was performed using

polymerase chain reaction (PCR) from isolated DNA samples. We found that IL- 1β levels

were significantly increased in the OCD group, whereas IL-10 was decreased (p < 0.001,

for both). Serum IL-4 levels and GSTM1 present/null genotype distributions were

comparable between groups (p > 0.05, for both). IL-10 levels were significantly different

in the OCD group carrying the GSTM1 null allele compared to the controls (p < 0.01).

Inflammation and having the GSTM null allele may contribute to the pathology of OCD.