Akademik Veri Yönetim Sistemi
Journal of Applied Research, cilt.4, sa.2, ss.336-345, 2004 (Scopus)
We developed an experimental animal model of posttraumatic pseudomonal endophthalmitis in rabbits to compare the relative efficacy of intravitreal ceftazidime and 3 doses of meropenem. A penetrating eye injury was made in the right eyes of 40 rabbits and 0.1 mL of 104 colony-forming units (cfu/mL) of Pseudomonas aeruginosa ATCC 27853 was injected into the midvitreous body the rabbits. Most animals showed the first signs of endophthalmitis 4 to 5 hours after the inoculation. The animals were examined clinically at 2, 4, 12, and 22 hours after surgery using slit-lamp biomicroscopy and indirect ophthalmoscopy. Rabbits were divided into 5 treatment groups of 8 rabbits; Group 1 received 0.1 mL intravitreal ceftazidime injection (22.5g/L); Group 2, 0.1 mL intravitreal injection of meropenem (0.5g/L); Group 3,0.1 mL intravitreal injection of meropenem (1g/L); Group 4,0.1 mL intravitreal injection of meropenem (2g/L); and Group 5, 0.1 mL intravitreal injection of normal saline. At 2 and 24 hours post treatment, two 100 μL vitreous samples were taken for high performance liquid chromatography analysis and colony counting in the culture. Vitreous levels of ceftazidime were above the 3 doses of meropenem at 2 hours post treatment. At 2 hours post treatment, there was no significant difference between the 3 different doses of meropenem (P< 0.01). There was no significant difference between vitreous levels of ceftazidime and the 3 doses of meropenem at 24 hours (P>0.05). Culture results showed no difference between treatment with ceftazidime and 3 doses of meropenem. Clinical and bacteriological examinations revealed significantly less inflammation in rabbits treated with ceftazidime and meropenem than saline control groups. Intravitreal antibiotic treatment both with ceftazidime and meropenem appears effective.