The establishment of dental implant stability is mandatory for successful osseointegration. Resonance frequency analysis (RFA) is the most frequently used method for the clinical measurement of implant stability. The purpose of the present study was to evaluate the reliability of the recently developed RF analyzer Penguin RFA and to compare it with the traditional RF analyzer Osstell ISQ. Sixty implants were inserted into fresh steer vertebrae and pelvis. Implant stability was measured using Penguin RFA by its transducers (multipegs) and Osstell ISQ by its transducers (smartpegs). Additionally, stability was measured by multipegs with Osstell ISQ and by smartpegs with Penguin RFA. The intraobserver and interobserver reliability of Penguin RFA were estimated by the intraclass coefficient (ICC). Mean implant stability quotients (ISQs) measured with Osstell ISQ were higher than the ISQs measured with Penguin RFA (P < .05). The intra- and interobserver reliability of Penguin RFA were considered as excellent (ICC > 0.7). For Osstell ISQ, no significance in ISQs was detected between the readings by smartpegs and multipegs (P > .05), while for Penguin RFA ISQs by smartpegs were significantly higher than the ISQs by multipegs (P <.05). The recently developed Penguin RFA is reliable and can be used in clinical practice for the measurement of dental implant stability regardless of the bone type. The multipegs originally manufactured for the Penguin RFA is also compatible with Osstell ISQ.