EFFECT OF TEMPOL ON REDOX HOMEOSTASIS AND STRESS TOLERANCE IN MIMETICALLY AGED DROSOPHILA
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, cilt.87, sa.1, ss.13-25, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 87 Sayı: 1
- Basım Tarihi: 2014
- Doi Numarası: 10.1002/arch.21176
- Dergi Adı: ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.13-25
- Anahtar Kelimeler: tempol, mimetic aging, Drosophila, oxidative stress, sialic acid, OXIDATION PROTEIN PRODUCTS, D-GALACTOSE, RENAL TISSUES, FREE-RADICALS, ACID, DYSFUNCTION, RATS, ANTIOXIDANTS, BIOMARKERS, DAMAGE
- İstanbul Üniversitesi Adresli: Evet
Özet
We aimed to test our hypothesis that scavenging reactive oxygen species (ROS) with tempol, a membrane permeable antioxidant, affects the type and magnitude of oxidative damage and stress tolerance through mimetic aging process in Drosophila. Drosophila colonies were randomly divided into three groups: (1) no D-galactose, no tempol; (2) D-galactose without tempol; (3) D-galactose, but with tempol. Mimetic aging was induced by D-galactose administration. The tempol-administered flies received tempol at the concentration of 0.2% in addition to D-galactose. Thiobarbituric acid reacting substance (TBARS) concentrations, advanced oxidation protein products (AOPPs), Cu, Zn-superoxide dismutase (Cu, Zn-SOD), sialic acid (SA) were determined. Additionally, stress tolerances were tested. Mimetically aged group without tempol led to a significant decrease in tolerance to heat, cold, and starvation (P < 0.05), but tempol was used for these parameters. The Cu, Zn-SOD activity and SA concentrations were lower in both mimetically aged and tempol-administered Drosophila groups compared to control (P < 0.05), whereas there were no significantly difference between mimetically aged and tempol-administered groups. Mimetically aged group without tempol led to a significant increase in tissue TBARS and AOPPs concentrations (P < 0.05). Coadministration of tempol could prevent these alterations. Scavenging ROS using tempol also restores redox homeostasis in mimetically aged group. Tempol partly restores age-related oxidative injury and increases stress tolerance. (C) 2014 Wiley Periodicals, Inc.