Probiotic-Enriched Live Food Improves Growth, Gut Health, and Fatty Acid Profile in Sobaity Seabream (Sparidentex hasta) Larvae
Aquaculture Research, cilt.2026, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 2026 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1155/are/6462306
- Dergi Adı: Aquaculture Research
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Artic & Antarctic Regions, BIOSIS, CAB Abstracts, Environment Index, Geobase, Directory of Open Access Journals, Zoological Record, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Earth, Atmospheric, & Aquatic Science Collection (ProQuest)
- Anahtar Kelimeler: digestive enzymes, fatty acid profile, larval growth, live food enrichment, probiotics, Sparidentex hasta
- İstanbul Üniversitesi Adresli: Evet
Özet
This study evaluated the effects of three probiotic strains (Bacillus subtilis, Lactobacillus plantarum, and Pediococcus acidilactici) on growth performance, survival rate, digestive physiology, antioxidant defense, fatty acid composition, and intestinal development of sobaity seabream (Sparidentex hasta) larvae over a 40-day rearing period. Larvae were assigned to five treatments: Control, Bacillus (Bac), Lactobacillus (Lac), Pediococcus (Pedi), and a mixed-probiotic group (Mix), and were fed rotifers and Artemia enriched with the corresponding probiotics. Probiotic supplementation significantly influenced larval performance and physiological markers across all treatments (p < 0.05). The Bac group exhibited significantly higher growth performance, reaching 15.62 ± 0.62 mm in length and 1072.13 ± 51.5 mg in weight by 40 days posthatch (p < 0.05). The Lac group also achieved a high final weight (1103.7 ± 153 mg; p < 0.05), albeit with a lower standard length (SL). Although the Pedi group demonstrated the highest digestive enzyme activity (e.g., lipase 1.39 ± 0.01 nmol/mg protein; p < 0.05) and antioxidant status (e.g., superoxide dismutase [SOD] 2.8 ± 0.02 nmol/mg protein; p < 0.05), these physiological improvements were not directly associated with superior growth performance. The mixed-strain group recorded the highest crude protein (21.41% ± 1.06%) and lipid (3.1% ± 0.87%) levels at 40 DPH (p < 0.05), despite exhibiting suboptimal weight gain. Fatty acid analysis revealed that the Bac treatment resulted in the highest docosahexaenoic acid (DHA) (1.91% ± 0.24%) and EPA (3.37% ± 0.07%) concentrations and the most favorable n − 3/n − 6 ratio (p < 0.05). Histological analysis revealed significant differences in intestinal morphology at 40 DPH (p < 0.05). The highest mucosal layer thickness (MLT) was observed in the Lac (12.23 ± 2.1 μm) group (p < 0.05), whereas the lowest value occurred in the Pedi group (4.67 ± 0.96 μm) (p < 0.05). Crypt depth (CD) was greatest in the Bac group (28.27 ± 2.88 μm), followed by Lac (16.71 ± 1.9 μm) and Pedi (14.52 ± 5.2 μm) (p < 0.05).These findings suggest that probiotic effects in larval sobaity seabream are strain-specific, with B. subtilis demonstrating the greatest potential to improve growth, nutrient assimilation, and early intestinal development in marine aquaculture systems.