Detection of the frequency of PER-1 type extended-spectrum beta-lactamase-producing Acinetobacter baumannii clinical isolates in Turkey: a multicenter study


Asik G., Ozdemir M., Kurtoglu M. G. , YAĞCI S., Oksuz L. , Gul M., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.44, ss.1041-1046, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 44 Konu: 6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/sag-1309-126
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Sayfa Sayıları: ss.1041-1046

Özet

Background/aim: beta-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type beta-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type beta-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type beta-lactamases in A. baumannii isolates in various regions of Turkey.
Aim: β-lactamases are an important resistance mechanism in Acinetobacter
baumannii. Pseudomonas extended-resistance (PER-1) type β-lactamase-producing
strains have been reported from various geographic locations; however, PER-1 type β-
lactamases from Turkish hospitals have not been investigated extensively. The aim of
this study was to determine the prevelance of PER-1 type β-lactamases in A. baumannii
isolates in various regions of Turkey.
Materials and Methods: A total of 763 clinical A. baumannii isolates were
collected from nine university hospitals and two state hospitals between 2008 and 2011.
Molecular amplification of the OXA-51 gene from the A. baumannii genome was
performed for in order to verify identification of the species . Real-time PCR were used
to detection of the blaPER-1 genes.
Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of
the PER-1 enzyme were detected 52.2%, 35.9%, and 8.3% in 2008, 2009 and 2010,
respectively. PER-1 prevalence decreased gradually during time. The differences
observed in PER-1 prevalence among the regions of Turkey were statistically
significant (χ2 test; P < 0.001).
Conclusion: This data demonstrate that the frequency of detection of PER-1 type
β-lactamases in A. baumannii species has decreased in our country. But, the increased
carbapenem resistance, together with multi drug resistance, has created a worrisome
situation regarding this pathogen.