Investigation of some DNA repair genes association in non small cell lung cancer.

Coskunpinar E. M., YILDIZ P., AYNACI E., Turna A., Oltulu Y. M., Hekimoglu E., ...Daha Fazla

Cellular and molecular biology (Noisy-le-Grand, France), cilt.61, sa.8, ss.57-62, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61 Sayı: 8
  • Basım Tarihi: 2015
  • Doi Numarası: 10.14715/cmb/2015.61.8.10
  • Dergi Adı: Cellular and molecular biology (Noisy-le-Grand, France)
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.57-62
  • Anahtar Kelimeler: rs13181 ERCC2 (T>G) (Lys751Gln), rs12806698 RRM1 (-269C>A), rs6759180 (located in the 5 ' UTR) RRM2 (10126436G>A), NSCLC, biomarker, MESSENGER-RNA EXPRESSION, MOLECULAR-BIOLOGY, XPD/ERCC2 POLYMORPHISMS, LYS751GLN POLYMORPHISM, EXCISION-REPAIR, RISK, GEMCITABINE, XRCC1, ERCC2, RRM1
  • İstanbul Üniversitesi Adresli: Evet

Özet

Ribonucleoside-diphosphate reductase subunit M2, also known as ribonucleotide reductase small subunit, is an enzyme that in humans is encoded by the RRM2 gene and also Ribonucleoside-diphosphate reductase large subunit is an enzyme that in humans is encoded by the RRM1 gene. RRM1 is a gene important in determining tumor phenotype, but also induced the expression of PTEN tumor suppressor gene, cell migration, invasion and metastasis formation, and play a preventive role. ERCC2 DNA repair mechanism is associated in more than 20 genes involved in the NER pathway. The aim of this study is to investigate rs13181 ERCC2 (T>G) (Lys751Gln), rs12806698 RRM1 (-269C>A) and rs6759180 (located in the 5'UTR) RRM2 (10126436G>A) gene polymorphisms by using real time PCR technique in patients with NSCLC. 193 NSCLC cases and 141 healthy control cases were included in this study. A significant difference was found between rs12806698 RRM1 genotype distributions (*p: 0.034) and were determined increases the risk of disease approximately 3.044 times AA genotype having (*p: 0.014 OR: 3.044, 95% CI: 1.205-7,688). A significant difference was found between rs6759180 RRM2 genotype distributions (*p: 0.033) and were determined increases the risk of disease approximately 3.49 times GG genotype having (p: 0,009 OR: 3, 49, % 95CI: 1.291-9,482). It was found significant difference in serum 8-OHdG levels between patients and controls (*p: 0001).