Research Information System
Uluslararası Katılımlı Türkiye VI. Bitki Koruma Kongresi, Konya, Turkey, 5 - 08 September 2016, pp.648, (Full Text)
Agrobacterium tumefaciens is a Gram negative bacterium responsible for causing crown gall disease
on host species by transferring and integrating its T-DNA into the plant genome. Over the past
decades, genetically engineered Agrobacterium has become one of the biotechnological tools capable
of generating transgenic plants. AtVIP1 (VirE2- Interacting Protein 1) is a bZIP transcription factor
characterized in Arabidopsis thaliana. Throughout Agrobacterium-mediated transformation, VIP1
protein interacts with the bacterial T-complex in the plant cytosol, by interacting with the VirE2
proteins. AtVIP1 then, via its Nuclear Localization Signal (NLS) domain, guide the T-complex into the
plant cell nucleus where it promotes the chromosomal integration of the target gene. In this study, we
have investigated AtVIP1 ortholog, HvVIP1 in barley plants, and analyzed its tissue-related
expression. HvVIP1 cDNA sequence was PCR-fished out from H. vulgare L., cv. Martı leaves, then
cloned into the pTZ57R/T cloning vector. Sequencing and blast analysis showed that HvVIP1 cDNA
has no similarity with the characterized AtVIP1 sequence. However, sequence similarity was found
with uncharacterized sequences from several cereal species including Brachypodium (87%), Zea Mays
(83%), Sorghum (82%) and Triticum aestivum (27%). HvVIP1 gene expression was also investigated
in barley plants and differential expresssion in leaves, roots, mature embryos, and calli was detected.
Detailed analysis of VIP1 which is an important factor in Agrobacterium infection, in monocots such
as barley can provide new knowledge on bacteria-host interactions and role of VIP1.