Background: Zinc is needed as essential for many physiological functions in humans and animals. Chitosan is a natural substance produced in the body from glucose and some biological effects such as antimicrobial and immune modification mechanisms. beta-glucans are polysaccharides of D-glucose monomers and have important roles in antitumor and antimicrobial activity in animals. Inulin is used for its effects on immune function, bioavailability of minerals, lipid metabolism and gastrointestinal tract health. The aim of the study was to investigate the effects of chitosan oligosaccharide (COS), beta-glucan, and inulin on some serum enzymes, metabolites and electrolytes in broilers.