A mutation screening and genotyping by high-resolution melting (HRM) to detect delta-6-desaturase (D-6-D) variants in Argania spinosa,

Louati M., UÇARLI C., ARIKAN B., Hannach, A. S. , Tetlow I., TURGUT KARA N.

2018 Eastern Regional Meeting, London, Canada, 24 November 2018, pp.32

  • Publication Type: Conference Paper / Full Text
  • City: London
  • Country: Canada
  • Page Numbers: pp.32
  • Istanbul University Affiliated: Yes


and in cosmetics, the tree is considered a good buffer against desertification and erosion and provides a
goodqualitywoodforvarious purposes.It’sraretofindthisspeciesoutsideofMorocco buthundreds
of treesexist in Tunisia indifferent locations. Fewargangenes aresequenced, and suchsequenced
study is to report the use of hight resolution melting (HRM) analysis for scanning and genotyping
Argania spinosa individuals which has never been reported before, to detect delta-6-desaturase (D-6-D)
variants. The present study describes an HRM analysis of natural sequence variation within a small
regionof D-6-Dgenefrom60argantreesbelongingtofourdifferentsitesinTunisia.MethylatedDNA
and unmethylated DNA acquire different sequences after bisulphite treatment resulting in PCR
various factors, including specificity and efficiency of PCR, amplicon length and position, and DNA
under investigation infactHRMclassified 10 groupsof variants carrying deletion mutants and single and multiple SNPs.