Investigation of the accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) in rat kidneys

Aydin H. , Ozdemir N., Uzunoren N.

FORENSIC SCIENCE INTERNATIONAL, vol.153, no.1, pp.53-57, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 153 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1016/j.forsciint.2005.04.018
  • Page Numbers: pp.53-57


The accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) and its metabolite 2,4-dichlorophenol (2,4-DCP) in the kidneys of rats was investigated. Male and female Sprague-Dawley rats were given 2,4-D in drinking water and food for 30 days. Group A (control group) was fed a normal diet, Group B Was fed 50 ppm 2,4-D in 15 g food, Group C received 100 ppm 2,4-D in 15 g food, Group D received 25 ppm 2,4-D in 15 ml drinking water and Group E was given 50 ppm 2,4-D in 15 ml of drinking water. Levels of 2,4-D and 2,4-DCP in kidneys were determined using high performance liquid chromatography (HPLC). It was observed that at low doses of 2,4-D, the metabolite, 2,4-DCP found in the kidneys. (C) 2005 Elsevier Ireland Ltd. All rights reserved.