Farklı fare ırklarında parthenogenotik oosit aktivasyonun in vitro gelişim oran ve kalitesinin karşılaştırılması
Veteriner Hekimler Derneği Dergisi, cilt.92, sa.1, ss.42-48, 2021 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 92 Sayı: 1
- Basım Tarihi: 2021
- Doi Numarası: 10.33188/vetheder.789268
- Dergi Adı: Veteriner Hekimler Derneği Dergisi
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.42-48
- İstanbul Üniversitesi Adresli: Evet
Özet
The aim of our research is to investigate the effects of parthenogenetic activation on in vitro embryo development rates
in different mouse strains. B6CBAF1, C57BL/6j, and B6D2F1 mouse strains were used in this study. Superovulated mice
were sacrificed and oocytes were obtained 14 hours after the human chorionic gonadotrophin (hCG) injection and the
parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated for 6 hours in 10 mM SrCl2
+ 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing Ca2+ free Chatot Ziomek Brinster (CZB)
activation medium. After this, further incubation was performed for two hours in an incubator at 37 °C and 5% CO2 in
embryo culturing medium + TSA. Finally, embryos were cultured for 120 hours. Parthenogenetic activation success of
the B6D2F1 mouse strain was found to be higher than C57BL/6j and B6CBAF1 strains