The Effect of Pirfenidone on Peyronie Plaques and Erectile Function in a Peyronie’s Rat Model

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Cevik G., Tozsin A., TAŞTEKİN E., Dursun M., AKTOZ T., Kadioglu A.

Journal of the College of Physicians and Surgeons Pakistan, vol.33, no.11, pp.1217-1222, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 11
  • Publication Date: 2023
  • Doi Number: 10.29271/jcpsp.2023.11.1217
  • Journal Name: Journal of the College of Physicians and Surgeons Pakistan
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1217-1222
  • Keywords: Anti-fibrotic agent, Erectile function, Experimental study, Peyronie’s disease, Pirfenidone
  • Istanbul University Affiliated: Yes


Objective: To investigate the anti-fibrotic effects of pirfenidone on Peyronie’s disease in an experimental rat model with intracavernosal injection of TGF-β and whether pirfenidone improves erectile function. Study Design: Experimental study. Place and Duration of the Study: Faculty of Medical Experimental Animals and Research Laboratory, Trakya University, from January to March 2021. Methodology: In this study, 27 male Sprague Dawley rats were used, and three groups were randomly identified. The rats in Group 1 served as the control group. Group 2 was not treated, and Group 3 was treated with pirfenidone therapy. The rats in Group 3 were administered pirfenidone 30 mg/kg/day by oral gavage, every day for four weeks, three weeks after the start of the experiment. At the end of seven weeks, a haemodynamic study was performed with cavernosal nerve stimulation to evaluate the erectile function, the rats were sacrificed, and the penile tissues were evaluated immunohistochemically. Results: MeICP/MIBP values were found to be higher in treated rats compared to rats in the untreated group but no statistically signifi-cant difference was found in MeICP/MIBP values between the control, Peyronie model, and treatment groups (p=0.25). According to the histopathological examination, the rate of fibrosis with H&E staining was mild (100%) in the control group, severe (100%) in the Peyronie group, and severe (87.5% severe and 12.5% moderate) in the Peyronie + treatment group. Conclusion: In the study, pirfenidone used in the treatment of Peyronie’s disease had a positive effect on erectile function, though not considered statistically significant. It has been shown that it has no histopathological effect on Peyronie’s plaques.