Production and characterization of alpha-galactosidase from Aspergillus flavipes


Ozsoy N. , Berkkan H.

CELL BIOCHEMISTRY AND FUNCTION, cilt.21, ss.387-389, 2003 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 21 Konu: 4
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1002/cbf.1041
  • Dergi Adı: CELL BIOCHEMISTRY AND FUNCTION
  • Sayfa Sayıları: ss.387-389

Özet

An extracellular a-galactosidase from the culture filtrate of Aspergillus flavipes grown on melibiose as a carbon source was partially purified by hydroxylapatite and diethylaminoethylcellulose chromatographies. Electrophoretic analysis showed protein bands corresponding to alpha-galactosidase and invertase activities. The optimum pH and temperature were determined as 4.5-5.0 and 45degreesC, respectively. The Km value for p-nitrophenyl-alpha-D-galactopyranoside was found to be 1.89 mm. The results reported in this study indicate that Aspergillus flavipes is indeed an active source of extracellular a-galactosidase. Copyright (C) 2003 John Wiley Sons, Ltd.