Production and characterization of alpha-galactosidase from Aspergillus flavipes

Ozsoy N., Berkkan H.

CELL BIOCHEMISTRY AND FUNCTION, vol.21, no.4, pp.387-389, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 4
  • Publication Date: 2003
  • Doi Number: 10.1002/cbf.1041
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.387-389
  • Istanbul University Affiliated: Yes


An extracellular a-galactosidase from the culture filtrate of Aspergillus flavipes grown on melibiose as a carbon source was partially purified by hydroxylapatite and diethylaminoethylcellulose chromatographies. Electrophoretic analysis showed protein bands corresponding to alpha-galactosidase and invertase activities. The optimum pH and temperature were determined as 4.5-5.0 and 45degreesC, respectively. The Km value for p-nitrophenyl-alpha-D-galactopyranoside was found to be 1.89 mm. The results reported in this study indicate that Aspergillus flavipes is indeed an active source of extracellular a-galactosidase. Copyright (C) 2003 John Wiley Sons, Ltd.