Update of the planktonic diatom genus pseudo-nitzschia in aotearoa new zealand coastal waters: Genetic diversity and toxin production

Nishimura T., Murray J. S., Boundy M. J., BALCI M., Bowers H. A., Smith K. F., ...Daha Fazla

Toxins, cilt.13, sa.9, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3390/toxins13090637
  • Dergi Adı: Toxins
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: amnesic shellfish poisoning, domoic acid, geographical distribution, liquid chromatography-tandem mass spectrometry, molecular phylogenetic analysis, Pacific Ocean, phytoplankton, DOMOIC ACID, SP-NOV, BACILLARIOPHYCEAE MORPHOLOGY, PUNGENS BACILLARIOPHYCEAE, COMPLEX BACILLARIOPHYCEAE, MOLECULAR PHYLOGENY, RAPID EXTRACTION, SHELLFISH, IDENTIFICATION, TOXICITY
  • İstanbul Üniversitesi Adresli: Evet

Özet

© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Domoic acid (DA) is produced by almost half of the species belonging to the diatom genus Pseudo-nitzschia and causes amnesic shellfish poisoning (ASP). It is, therefore, important to investigate the diversity and toxin production of Pseudo-nitzschia species for ASP risk assessments. Between 2018 and 2020, seawater samples were collected from various sites around Aotearoa New Zealand, and 130 clonal isolates of Pseudo-nitzschia were established. Molecular phylogenetic analysis of partial large subunit ribosomal DNA and/or internal transcribed spacer regions revealed that the isolates were divided into 14 species (Pseudo-nitzschia americana, Pseudo-nitzschia arenysensis, Pseudo-nitzschia australis, Pseudo-nitzschia calliantha, Pseudo-nitzschia cuspidata, Pseudo-nitzschia delicatissima, Pseudo-nitzschia fraudulenta, Pseudo-nitzschia galaxiae, Pseudo-nitzschia hasleana, Pseudo-nitzschia multiseries, Pseudo-nitzschia multistriata, Pseudo-nitzschia plurisecta, Pseudo-nitzschia pungens, and Pseudo-nitzschia cf. subpacifica). The P. delicatissima and P. hasleana strains were further divided into two clades/subclades (I and II). Liquid chromatography-tandem mass spectrometry was used to assess the production of DA and DA isomers by 73 representative strains. The analyses revealed that two (P. australis and P. multiseries) of the 14 species produced DA as a primary analogue, along with several DA isomers. This study is the first geographical distribution record of P. arenysensis, P. cuspidata, P. galaxiae, and P. hasleana in New Zealand coastal waters.