Can novel methods replace the gold standard chimerism method after allogeneic hematopoietic stem cell transplantation?

Sel F. A., Oğuz F. S.

Annals of Hematology, cilt.103, sa.4, ss.1035-1047, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 103 Sayı: 4
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s00277-023-05448-3
  • Dergi Adı: Annals of Hematology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, CINAHL, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.1035-1047
  • Anahtar Kelimeler: Chimerism monitoring, Dd-PCR, Molecular diagnostics, Multiplex STR-PCR, NGS, qPCR
  • İstanbul Üniversitesi Adresli: Evet

Özet

After hematopoietic stem cell transplantation, chimerism assay is a useful approach to monitor the success of the transplant and to select the appropriate treatment strategy, such as donor leukocyte infusion or immunosuppressive drug dosage. Short tandem repeat PCR is the method that has been accepted as the gold standard for chimerism. However, it has not yet been sufficient to detect mixed chimerism in patients with minimal residual disease. Simultaneously, recent years have been marked by developing sensitive, high-throughput, and accurate molecular genetic assays. These novel methods have subsequently been adapted for the analysis of post-transplant chimerism. In this review, we discuss the technical features of both novel and conventional gold standard chimerism assays. We also discuss their advantages and disadvantages.