PTEN gene expression on canine mammary tumors

Altun E. D., FIRAT İ., KUŞKUCU M. A.

2nd Turkish-Bosnian Scientific Days, Turkey, 1 - 04 May 2013, pp.54

  • Publication Type: Conference Paper / Full Text
  • Country: Turkey
  • Page Numbers: pp.54
  • Istanbul University Affiliated: Yes



Objectives: Studies on molecular basis are continued to be performed in order to determine the factors effecting the development mechanism, prognosis and treatment of canine mammary tumors, which are very common at the present time. Within this framework the roles of anti-oncogenes on tumor development were tried to be revealed with many studies. In this study the expression of PTEN genes in normal mammary gland and tumor tissues and its availability as an auxiliary factor were determined.


Material and methods: PTEN gene expression levels were determined with Real-time Polymerase Chain Reaction (RT-PCR) in 32 malignant tumoral and 20 normal mammary gland tissues which were collected from dogs that had total mastectomy and histologic classification.  Beside the expression of PTEN gene in normal and tumoral tissues, the histologic type of tumoral cases, tumor sizes, grades, the animal's age, breed were compared with ovario-hysterectomie and birthing age.


Results: The PTEN gene levels were found to be 1.5 times lower in tumoral tissues, but the difference was statistically insignificant (p>0.05). When the tumors were compared regarding to histologic types, tumor sizes, grades, the animal's age, breed, ovario-hysterectomie and birthing age it was determined that there were significance but none of them were statistically meaningful (p<0.05). Nevertheless in cases where lymphoid metastases were observed significant decrease in PTEN gene expression was determined (p<0.05). This finding showed that the decreases in PTEN gene expression have an important effect in aggressive and metastatic mammary tumors.


Conclusion: We think that to understand the effect of PTEN gene on tumor pathogenesis and to evaluate its auxiliary prognostic value different studies which determines the PTEN gene expression in different dog breeds, different histomorphologic tumors and which reveals the PTEN gene expression scale in the basis of breeds should be developed.



Key words: Canine, Mammary tumors, PTEN gene, RT-PCR