Akademik Veri Yönetim Sistemi
9th International Congress of the Molecular Biology Association of Turkey, İzmir, Türkiye, 12 - 14 Eylül 2024, ss.77
Background/Aim: Dextran sulfate sodium (DSS) is often preferred to induce an ulcerative colitis
model in experimental animals. DSS affects the small intestine as well as the colon. Suramin, used as
a therapeutic agent in African trypanosomiasis, is a histone deacetylase inhibitor that can inhibit NAD+-
dependent histone deacetylases and exhibits broad biological effects, including antiproliferative
and antiviral activities. In our study, we aimed to investigate the antioxidant and anti-inflammatory
effects of suramin on the intestinal injury caused by DSS in mice.
Materials and Methods: In our study, 32 C57BL/6 mice were divided into four groups. DSS group
mice were administered 3% DSS dissolved in drinking water for five days. Suramin was administered
intraperitoneally at a dose of 25 mg/kg for seven days. Malondialdehyde (MDA) and glutathione
(GSH) levels, as well as myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD), and
glutathione peroxidase (GPx) activities, were determined spectrophotometrically in intestinal tissues.
Expression levels of cyclooxygenase-2 (COX-2) and proinflammatory cytokines, such as tumor necrosis
factor alpha (TNF-α) and interleukin-6 (IL-6), were determined by western blotting.
Results: DSS application caused oxidative damage in the intestinal tissues by increasing MDA levels
and MPO activity, and decreasing GSH levels and CAT, SOD, and GPx activity. Additionally, DSS
stimulated inflammation by increasing COX-2, TNF- α, and IL-6 levels. Injection of suramin into
DSS-given animals significantly increased in intestinal GSH levels and antioxidant enzyme activities
while reducing the elevated MDA levels. Suramin prevented the intestinal injury in the DSS-induced
colitis model by decreasing COX-2, TNF- α, and IL-6 expression levels.
Conclusion: This study showed that suramin has antioxidant and anti-inflammatory effects on the
small intestine in a mouse colitis model. The protective effects of suramin observed intestinal tissue
suggest that it may be a useful therapeutic agent for inflammatory bowel diseases.