Turn-on fluorometric assay based on functionalized gold nanoparticles for measurement of nitric oxide radical scavenging activity of phenol-, amine-, and thiol-type antioxidants

Ermiş S., Türk Ş., KOÇ K. H., ARDA A., APAK M. R.

Food Chemistry, cilt.493, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 493
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.foodchem.2025.146108
  • Dergi Adı: Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Antioxidants, Gold nanoparticle, N-(1-naphthyl) ethylenediamine, Nitric oxide radical, Scavenging activity, Turn-on fluorescence
  • İstanbul Üniversitesi Adresli: Hayır

Özet

An innovative fluorometric method has been developed to detect nitric oxide radicals (•NO) and NO-scavenging activity of antioxidants. Nitric oxide radicals were generated from the precursor sodium nitroprusside (SNP), and gold nanoparticles modified with a thiolate ligand (4-hydroxythiophenol) (AuNPs@4-HTP) was designed as the detector probe; addition of •NO to the thiophenol ring of AuNPs@4-HTP was followed by autoxidation of the NO-substituent to -NO2 which in turn interacted with the fluorophore N-(1-naphthyl)ethylenediamine (NEDa) as a Lewis acceptor-donor pair. As a result of the interaction of nitrated AuNPs@4-HTP(ox) with the amine groups of NEDa, the fluorescence of NEDa was quenched. However, antioxidants added to the probe solution scavenged •NO, thus the non-nitrated AuNPs@4-HTP could not interact with NEDa, and the fluorescence of the probe solution was recovered, with simultaneous measurement of the •NO scavenging activity of the tested antioxidants μmol L−1 levels. The method gave good recovery from food samples.