The effect of interleukin (IL)-21 and CD4(+)CD25(++) T cells on cytokine production of CD4(+) responder T cells in patients with myasthenia gravis

Alahgholi-Hajibehzad M., Durmuş H., AYSAL F., Guelsen-Parman Y., Oflazer P., Deymeer F., ...Daha Fazla

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, cilt.190, sa.2, ss.201-207, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 190 Konu: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1111/cei.13006
  • Sayfa Sayıları: ss.201-207


Impairment of the suppressive function of regulatory T (T-reg) cells has been reported in myasthenia gravis (MG). In this study, cytokine-related mechanisms that may lead to the defect of T-reg were investigated in patients with anti-acetylcholine receptor antibody-positive MG (AChR+MG). Proliferation and cytokine production of responder T (T-resp) cells in response to polyclonal activation were measured in a suppression assay. The effect of interleukin (IL)-21 on suppression was evaluated in vitro in co-culture. IL-21 increased the proliferation of T-resp cells in T-resp/T-reg co-cultures. T-resp cells from patients with MG secreted significantly lower levels of IL-2. In patients with MG, IL-2 levels did not change with the addition of T-reg to cultures, whereas it decreased significantly in controls. In T-resp/T-reg co-cultures, IL-4, IL-6 and IL-10 production increased in the presence of T-reg in patients. Interferon (IFN)-gamma was decreased, whereas IL-17A was increased in both patient and control groups. IL-21 inhibited the secretion of IL-4 in MG and healthy controls (HC), and IL-17A in HC only. The results demonstrated that IL-21 enhances the proliferation of T-resp cells in the presence of T-reg. An effect of IL-21 mainly on T-resp cells through IL-2 is implicated.