III. International Biological & Life Sciences Congress, Antalya, Türkiye, 16 - 19 Kasım 2025, cilt.1, ss.195, (Özet Bildiri)
Nanotechnology and phytohormone applications offer innovative strategies for promoting plant growth. Indole-3-acetic acid (IAA) is a core phytohormone involved in cell division and elongation, while zinc oxide nanoparticles (ZnO NPs) synthesized via green methods using Acanthus spinosus L. leaf extract, can enhanced the growth. This study investigated the effects of single and combined applications of IAA and biosynthetic ZnO NPs on growth promotion in Lepidium sativum L. seedlings. Seven-day-old of Lepidium sativum seedlings were organised as The control, IAA, ZnO NPs, and IAA + ZnO NPs treatment groups. Growth parameters, Pigment contents, secondary metabolites, and enzyme activities were analyzed. The results are showed that both IAA and ZnO NPs applications enhanced growth and pigmentation, with the highest values obtained in the combined application. Proline supports osmotic balance, while increases in POD and CAT activities have been linked to increased ROS signaling during growth. Furthermore, ZnO NPs were determined to be biocompatible at low concentrations. Our study demonstrates that the combined use of phytohormone and nanoparticle applications contributes an effective approach to promoting plant growth and enhancing physiological responses.