Coupling dalapon biodegradation with electricity generation in microbial fuel cells

Sarıoğlu Y., Sever Kaya D., Kurt H., Kantarci-Carsibasi N., Kul A., Maraklı S., ...More

BIORESOURCE TECHNOLOGY, vol.445, 2026 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 445
  • Publication Date: 2026
  • Doi Number: 10.1016/j.biortech.2026.134073
  • Journal Name: BIORESOURCE TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Compendex, EMBASE, Environment Index, Geobase, INSPEC, MEDLINE
  • Istanbul University Affiliated: Yes

Abstract

Dalapon (2,2-dichloropropionic acid) is a persistent halogenated herbicide frequently detected in aquatic environments, yet its bioelectrochemical degradation has not been previously demonstrated. This study reports the simultaneous biodegradation of dalapon and electricity generation in single-chamber microbial fuel cells inoculated with the psychrotolerant Antarctic isolate Psychrobacter sp. TaeBurcu001. While mixed microbial cultures alone were unable to oxidize dalapon as the sole added carbon source, co-inoculation with TaeBurcu001 enabled measurable electricity generation (0.1-0.21 V at 980 Omega) and achieved more than 90% dalapon removal. Targeted LC-MS/MS analysis confirmed substantial dalapon degradation under all tested conditions. Microbial community analysis based on 16S rRNA gene sequencing revealed enrichment of electrogenic and xenobiotic-degrading genera, including Xanthobacter, Pseudomonas, Achromobacter, and Dysgonomonas. Molecular docking and molecular dynamics simulations suggested favorable binding of dalapon within the catalytic pocket of L-2-haloacid dehalogenase, supporting a plausible enzymatic contribution to dehalogenation. Overall, this study demonstrates