Skin and subcutaneous tissue tumors are the most common neoplasms in dogs. The most common sites of origin in dogs include digits, skin and the oral cavity including cheek and retromandibular area. We investigated canine squamous cell carcinoma (SCC) samples from 15 dogs and classified them histopathologically according to the degree of differentiation. bFGF, VEGF-C, TGF-beta, PDGF-A, PDGF-C and PDGFR-alpha expressions were assessed using immunohistochemistry to determine the roles of growth factors during SCC. We found that TGF-beta 1 immunolabeling was elevated significantly compared to healthy controls in SCC originating from nailbeds, while expression of other growth factors did not change significantly. Our findings might explain the role of TGF-beta 1 in the infiltrative and malignant behavior of SCC originating from nailbeds.