Research Information System
İstanbul Üniversitesi İstanbul Tıp Fakültesi Mecmuası, vol.88, no.1, pp.60-71, 2025 (Scopus, TRDizin)
ABSTRACT
Objective: S-adenosylmethionine (SAM) has antioxidant and anti-inflammatory actions and hepatoprotective potential. In this study, the therapeutic effectiveness of SAM was investigated in high-fat/cholesterol diet (HFCD)-induced non-alcoholic steatohepatitis (NASH).
Material and Methods: In this study, guinea pigs received a HFCD for ten weeks to develop NASH. SAM (50 mg/kg, i.p) was applied to animals in the last four weeks of the 10-week HFCD application. Hepatic damage markers, lipid levels (total cholesterol and triglyceride), inflammatory cytokines (tumor necrosis-α and interleukin-6) levels, and insulin resistance (HOMA-IR) were determined in the serum. Moreover, hepatic lipids, SAM and cytochrome p450-2E1 (CYP2E1) levels, prooxidant parameters (reactive oxygen species, lipid peroxides, and protein carbonyls), and antioxidant parameters (glutathione levels and antioxidant activity) together with fibrosis indicators (α-smooth muscle actin and transforming growth factor-β1 protein expressions and hydroxyproline levels) were investigated in the liver. Steatosis, inflammation, and fibrosis scores were also detected histopathologically.
Result: SAM treatment diminished the increase in hepatic damage markers, inflammatory cytokine levels, and HOMA-IR levels.