Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: optimisation and cellular studies

Cevher E., Salomon S. K., Makrakis A., Li X. W., Brocchini S., Alpar H. O.

JOURNAL OF MICROENCAPSULATION, vol.32, no.8, pp.755-768, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 8
  • Publication Date: 2015
  • Doi Number: 10.3109/02652048.2015.1073392
  • Journal Name: JOURNAL OF MICROENCAPSULATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.755-768
  • Keywords: Carboxymethyl pullulan, cellular uptake, chitosan derivatives, nanoparticles, nasal vaccination, N-trimethyl chitosan chloride, polyion complexation, N-TRIMETHYL CHITOSAN, MOLECULAR-WEIGHT, HEPATITIS-B, IN-VITRO, POLYELECTROLYTE COMPLEXES, INTRANASAL VACCINATION, BIOLOGICAL-PROPERTIES, ALVEOLAR MACROPHAGES, ABSORPTION ENHANCER, ACID NANOPARTICLES
  • Istanbul University Affiliated: Yes

Abstract

Nasal immunisation with nanoparticles has already shown promising results. In this study, nanoparticle composites carrying BSA for nasal vaccination prepared using electrostatic interaction process between polycation N-trimethyl chitosan chloride (TMC), chitosan glutamate (CG), chitosan chloride (CCl) and polyanion carboxymethyl pullulan (CMP). A mass ratio of 2:1 for TMC-CMP combination produced stable nanocarriers. For CCl-CMP and CG-CMP formulations needed a mass ratio of 3:1. Loading efficiency was >90% for all formulations. Nanoparticles' size ranged from 207 to 603 nm. The surface charge of the complexes varied between +14 and +33 mV. SDS-PAGE integrity of the model antigen was also demonstrated. MTT studies showed that nanoparticle composites were less toxic to Calu-3 cells than the particles of cationic polymers alone. FITC-BSA loaded nanoparticles efficiently taken up by J774A.1 macrophages as confirmed by confocal microscopy highlighting the potential of these novel nanoparticulate carriers' use for nasal vaccination.