Mycoplasma hyopneumoniae is the primary agent of enzootic pneumonia in pigs. The aim of this study was to investigate the occurrence of M. hyopneumoniae in pigs raised in Turkey. Samples were obtained from the lungs of 286 pigs, which had already been separated from the carcasses at slaughter. Lung tissues were homogenized in 5 ml of Friis medium, the suspensions were inoculated into Friis medium, and then incubated at 37 degrees C until they developed an acid color change or for 21 days. Cultures were identified by M. hyopneumoniae-specific PCR. M. hyopneumoniae was recovered from 2 (0.70%) of the 286 lung samples. This study is the first to report the isolation of M. hyopneumoniae in pigs in Turkey.