Antagonistic contact on tips of active micro forceps produces surface stresses leading to fracture and wear finally leading to fatal failure. It was the aim of the present research to study the outcome of low cycle loading testing parts of active micro forceps materials involving either surface contact fatigue or flexural loading mechanisms. For this purpose, this research was focused on the mechanisms of the fatigue life of 316 L-type stainless steel active micro forceps in low cycle loading conditions. This could result in the fatigue failure of active micro forceps at stress levels below the yielding stress of material. Thus, researching the material and mechanical behaviors of an active micro forceps structure and force mechanism under low cycle loading is vital. Finite element method with accurate geometry and material properties was employed for a biocompatible forceps' tips in the computational modeling. To justify the data collected from Von Mises' yield condition, the Haigh diagram was developed to analyze fatigue wear. The low cycle loading behavior of the active micro forceps was analyzed in computational engineering tool of ANSYS LS-DYNA under operational load conditions in vitrectomy. The results of the analysis obtained from this research are helpful for micro component manufacturer and clinic surgery operation.