Relationship between CD107a expression and cytotoxic activity

Aktas E., Kucuksezer U. C., Bilgic S., Erten G., Deniz G.

CELLULAR IMMUNOLOGY, vol.254, no.2, pp.149-154, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 254 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1016/j.cellimm.2008.08.007
  • Journal Name: CELLULAR IMMUNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.149-154
  • Keywords: NK cells, CD107a, Cylotoxicity, CFSE, IL-2, IFN-gamma, TNF-alpha, IL-10, NATURAL-KILLER-CELLS, HUMAN NK CELLS, SECRETORY LYSOSOMES, TARGET-CELLS, ASSAY, DIFFERENTIATION, IDENTIFICATION, GLYCOPROTEINS, ACTIVATION, CHEMOKINES
  • Istanbul University Affiliated: Yes

Abstract

NK cells play important roles in innate immunity against tumors and infections of the host. Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8(+) T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56(+) NK, CD8(+) T cells and lymphocytes has been determined after various stimuli. Effector cells from PBMCs of healthy subjects were isolated and K562 cell line was used as target of cytotoxicity. IL-2 stimulation resulted in a significant increase of CD107a expression in CID56(+) NK, CD8(+) T cells and lymphocytes. Increased expression of CD107a after IL-2 stimulation of NK cells was parallel to the increase of cytotoxicity. Our results suggest that CD107a expression may be a sensitive marker for the cytotoxic activity determination. (c) 2008 Elsevier Inc. All rights reserved.