Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Akaylı T., Timur G., Albayrak G., Aydemir B.

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, cilt.62, sa.1, ss.9-18, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 1
  • Basım Tarihi: 2010
  • Dergi Adı: ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.9-18
  • Anahtar Kelimeler: gilthead sea bream, Vibrio ordalii, serology, SDS-PAGE, RAPD, MARINE FISH, ANGUILLARUM, PATHOGENS, PCR
  • İstanbul Üniversitesi Adresli: Evet

Özet

The phenotypical, serological, and molecular identification of Vibrio ordalii isolates recovered from diseased gilthead sea bream (Sparus aurata) in the Mediterranean area were investigated. Most isolates had similar phenotypic characteristics, but some showed small differentiation in biochemical analysis. Both slide agglutination and enzyme linked immunosorbent assay (ELISA) showed that the isolates were antigenically homogeneous and no different serotypes were detected. SDS-PAGE analysis showed that the structure of lipopolysaccharide (LPS) was heterogeneous, that outer membrane proteins (OMP) display homogeneous grouping protein banding patterns, and that the LPS profile of the isolates had different banding patterns. However, random amplified polymorphic DNA (RAPD) analysis revealed genetic variation (up to 90.4%) among isolates of the same species.