Akademik Veri Yönetim Sistemi
International Congress On Natural Products, Krakow, Polonya, 13 - 17 Temmuz 2024, ss.616, (Özet Bildiri)
Taraxacum aleppicum belongs to the genus Taraxacum which is a member of the Asteraceae family. The
genus Taraxacum is found in warmer places of the Northern Hemisphere and it is represented in Turkiye as
54 taxa under nine sections (Uysal et al., 2016). T. aleppicum is a member of the Scariosa section which is
characterized by a reddish, brown or straw-coloured achene and is used for eye diseases in animals (Gürdal,
2015, Selvi et al., 2023). As a part of our current study, T. aleppicum was collected from Sakarya in Marmara
region, Turkiye. Air-dried roots and aerial parts were separated and extracted with ethanol. Ethanol extracts
of roots and aerial parts were partitioned with petroleum ether, ethyl acetate and n-butanol, respectively.
The chemical composition of root and aerial part fractions was elucidated using LC-HRMS. The main
components of the root EtOAc fraction are chichoric acid, sinapinic acid and chlorogenic acid, respectively.
The EtOAc fraction of the aerial part contain mainly chichoric acid, chlorogenic acid and luteolin-7-
rutinoside. The main component of BuOH fractions of both root and aerial parts was found to be chichoric
acid. The antimicrobial activity of both root and aerial parts fractions was examined against standard strains
using the minimum inhibitory concentration (MIC) method (CLSI, 2000 & 2006).
Table 1. Antimicrobial activities of the fractions (μg/mL). PE: Petroleum ether, EtOAc: Ethyl acetate, BuOH:
Butanol, ap. fr.: aerial part fraction, ro. fr.: root fraction, S.a.: Staphylococcus aureus, S.e.: Staphylococcus
epidermidis, K.p.: Klebsiella pneumonia, E.c.: Escherichia coli, P.a.: Pseudomonas aeruginosa, C.a.: Candida
albicans, C.t.: Candida tropicalis, C.p.: Candida parapsilosis, ATTC: American Type Culture Collection
Strains Samples
S.a.
ATCC
29213
S.e.
ATCC
12228
K.p.
ATCC
4352
E.c.
ATCC
25922
P.a.
ATCC
27853
C.a.
ATCC
10231
C.t.
ATCC
750
C.p.
ATCC
22019
PE ap. fr. 625 72.8 1250 625 625 312.5 625 625
EtOAc ap. fr. 625 156.25 1250 625 625 312.5 625 625
BuOH ap. fr. 625 312.5 625 1250 625 312.5 625 625
PE ro. fr. 625 625 625 1250 625 312.5 312.5 625
EtOAc ro. fr. 1250 625 625 625 625 312.5 625 625
BuOH ro. fr. 625 625 625 1250 625 625 312.5 625
According to the results (Table 1), while root fractions of T. aleppicum were found to be moderately effective
against all standard strains, fractions of aerial parts were found to be very effective against Staphylococcus
epidermidis ATCC 12228.
Keywords: Taraxacum aleppicum, antimicrobial, LC-HRMS
References
Clinical and Laboratory Standards Institute (CLSI), 2000., Reference method for broth dilution antifungal susceptibility testing of yeasts;
Approved Standard M27-A NCCLS, Wayne, Pennsylvania.
Clinical and Laboratory Standards Institute (CLSI), 2006., Methods for dilution antimicrobial susceptibility tests for bacteria that grow
aerobically: Approved Standard M7-A5. Wayne, PA: CLSI.
Gürdal, B., 2015. Pharmaceutical botany investigations on taraxacum species growing in marmara region. Doctoral dissertation, Istanbul
University.
Selvi, S., Koç, F.A., Satıl, F. 2023. An ethnoveterinary study on plants used for in the treatment of livestock diseases in Ayvalık (Balıkesir, Turkey).
Indian J. Nat. Prod. Resour. (IJNPR) [Formerly Natural Product Radiance (NPR)]. 14(2), 300-312.
Uysal, A., Zengin, G., Gunes, E., Uysal, N., 2016. Natural two Taraxacum species effective against frame shift and base pair substitution mutations:
mutagenic, antimutagenic and antioxidant evaluation. Marmara Pharm J. 20(3), 311-319.