Protamine gold nanoclusters - based fluorescence turn-on sensor for rapid determination of Trinitrotoluene (TNT)

Bener M., Sen F. B., APAK M. R.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, cilt.279, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 279
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.saa.2022.121462
  • Dergi Adı: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, INSPEC, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Trinitrotoluene (TNT) determination, Nitroaromatic explosives, Gold nanoclusters, Fluorescence enhancement, Protamine, ELECTROCHEMICAL SENSOR, COPPER NANOCLUSTERS, ENERGETIC MATERIALS, EXPLOSIVES, 2,4,6-TRINITROPHENOL, NANOPARTICLES, RECOGNITION, NANOSENSOR, POLYMER, DNT
  • İstanbul Üniversitesi Adresli: Evet

Özet

Determination of trace residues of 2,4,6-trinitrotoluene (TNT) is an analytical challenge as it is widely used in military, mining industry, civilian and counter-terrorism purposes. In this study, a gold nanocluster based turn on fluorescence sensor was developed for TNT determination. A one-pot approach was used to synthesize the fluorescent protamine stabilized gold nanoclusters (PRT-AuNC). The proposed turn-on fluorometric sensor relies on the aggregation-induced emission enhancement mechanism. As a result of the donor-acceptor interaction between the non-fluorescent Meisenheimer anion formed from TNT and the amino groups of weakly fluorescent protamine, the PRT-AuNCs aggregate and an accompanying enhancement in fluorescence intensity is observed with a large Stokes shift (lambda(ex) = 300 nm, lambda(em) = 600 nm). The fluorescence enhancement increased linearly with TNT with an LOD of 12.44 mu g/L. Similar energetic materials, common soil ions and explosive camouflage materials did not affect the proposed fluorometric sensing method. TNT in artificially contaminated soil was determined, and the results were comparable to those obtained by the HPLC-DAD system. The proposed turn-on sensor is an important tool for simple, fast, rapid and sensitive TNT determination, and has a potential to be converted to a kit format.