Selective and sensitive detection of glutathione with a novel fluorescein-based probe in fully aqueous media and its application in food samples

Suna G., Topal S., Karakuş E.

Dyes and Pigments, vol.234, 2025 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 234
  • Publication Date: 2025
  • Doi Number: 10.1016/j.dyepig.2024.112551
  • Journal Name: Dyes and Pigments
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: Biothiols, Fluorescein, Fluorescent probe, Fruit and vegetable sample analysis, Glutathione (GSH) detection
  • Istanbul University Affiliated: No

Abstract

We devised a novel fluorescent probe, FL–T, bearing fluorescein and thienyl acrylic acid moiety, which was designed and synthesised to detect glutathione (GSH). Utilising micelle-catalysed cyclisation/elimination reaction, FL–T demonstrated exceptional sensitivity and operability in a fully aqueous solution, with a low limit of detection (LOD = 41.2 nM), and superior selectivity over cysteine (Cys), homocysteine (Hcy), H2S and other analytes. Furthermore, FL–T successfully detected GSH in fruit and vegetable samples. These findings suggest that FL–T is a promising tool for detecting GSH in various environments.