Selective and sensitive detection of glutathione with a novel fluorescein-based probe in fully aqueous media and its application in food samples

Suna G., Topal S., Karakuş E.

Dyes and Pigments, cilt.234, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 234
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.dyepig.2024.112551
  • Dergi Adı: Dyes and Pigments
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Biothiols, Fluorescein, Fluorescent probe, Fruit and vegetable sample analysis, Glutathione (GSH) detection
  • İstanbul Üniversitesi Adresli: Hayır

Özet

We devised a novel fluorescent probe, FL–T, bearing fluorescein and thienyl acrylic acid moiety, which was designed and synthesised to detect glutathione (GSH). Utilising micelle-catalysed cyclisation/elimination reaction, FL–T demonstrated exceptional sensitivity and operability in a fully aqueous solution, with a low limit of detection (LOD = 41.2 nM), and superior selectivity over cysteine (Cys), homocysteine (Hcy), H2S and other analytes. Furthermore, FL–T successfully detected GSH in fruit and vegetable samples. These findings suggest that FL–T is a promising tool for detecting GSH in various environments.