Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Türkiye, 23 - 25 Kasım 2022, ss.1730, (Özet Bildiri)
During carcinogenesis, epigenetic alterations contribute to malignant properties that leads to inappropriate silencing or
activation of cancer-associated genes. There are limited number of studies investigating the relationship of a slight
increase in the level of ROS production in cancer stem cells with the regulation of DNA methylation. We aimed to
investigate whether a slight increase in ROS levels changes methylation status at CpG sites in the promoters of more than
200 genes related to stemness, metabolism, signaling pathways, epithelial-mesenchymal transition, drug transporters,
antioxidant enzymes, and glycosyltransferases. PANC-1 cells were treated with 0.1 mM H2O2 for 24h and cancer stem
cells were sorted by their ALDEFLOUR positivity. After DNA isolation, methylated DNA was immunoprecipitated and
labeled. Methylated promoters were detected by microarray and analyzed bioinformatically. Besides any changes in
global DNA methylation were also researched following H2O2 treatment. The promoters of genes related to Wnt and
Sonic hedgehog (Shh) pathways were differently methylated in cancer stem cells exposed to 0.1 mM H2O2. The levels of
5-mC increased in the experimental group compared to the control. These results suggest that slight increase in ROS level
may lead to epigenetic changes via DNA methylation in pancreatic cancer stem cells and effect the properties of stemness.
Keywords: Cancer stem cell, reactive oxygen species, stemness, MeDIP array, global DNA methylation.