Concordance of PD-L1 expression and CD8(+) TIL intensity between NSCLC and synchronous brain metastases


BATUR Ş., Dulger O., DURAK Ş., Yumuk P. F. , Caglar H. B. , BOZKURTLAR E., ...More

BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol.20, no.3, pp.329-335, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.17305/bjbms.2019.4474
  • Title of Journal : BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
  • Page Numbers: pp.329-335

Abstract

Programmed death-ligand 1 (PD-L1) is suggested to be a predictive biomarker in non-small-cell lung carcinoma (NSCLC). However, the differential expression of PD-L1 in primary lung tumor vs. synchronous metastases, especially brain metastasis (BM), remains unclear. This study assessed the concordance of PD-L1 expression on tumor cells and tumor-infiltrating lymphocytes (TILs) and CD8(+) TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values <1%, 1-49%, >= 50%. CD3 and CD8 positivity in TILs was evaluated semi-quantitatively and the proportion of CD3+/CD8(+)TILs was determined. PD-L1 expression on tumor cells and TILs was evaluated in relation to CD3+/CD8(+)TIL proportions and the intensity of CD8(+)TILs between the paired primary lung and BM tissues. In the primary lung tumors, PD-L1 positivity was observed in 25%, 37.5%, and 37.5% cases for the cutoff values <1%, 1-49%, >= 50% respectively. PD-L1 expression on tumor cells was strongly correlated between the paired primary lung and BM tissues, in all cutoff groups. However, PD-L1 expression on TILs and the proportion of CD3+/CD8(+)TILs were not strongly correlated in all three groups between the paired primary lung tumors and BMs. The intensity of CD8(+)TILs was concordant in only 54.16% of the paired primary lung tumors and BMs. This study showed a high concordance of PD-L1 expression in neoplastic cells between primary NSCLC and synchronous BMs.