Analysis of hepatitis C virus NS5A region in patients with cirrhosis using an ultra-deep pyrosequencing method

Keskin F., Ciftci S., Akyuz F., Abaci N., Cakiris A., Akyuz U., ...Daha Fazla

Clinical Laboratory, cilt.63, sa.9, ss.1439-1445, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 9
  • Basım Tarihi: 2017
  • Doi Numarası: 10.7754/clin.lab.2017.170312
  • Dergi Adı: Clinical Laboratory
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1439-1445
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background: HCV (Hepatitis C Virus) is genetically more diverse than HBV and HIV (Human Immunodeficiency Virus) and exists as quasispecies within infected individuals. This is due to the lack of efficient proofreading of the viral RNA-dependent RNA polymerase. Consequently, quasispecies emerge depending on the mutation rate of the viral polymerase, which may display a high level of genetic variability in a population. In infected individuals, HCV replicates and circulates as quasispecies composed of a complex mixture of different but closely related genomes that undergoes continuous change due to competitive selection and cooperation between arising mutants. The aim of this study is to investigate mutations in the NS5A region as a whole, including ISDR, PKRBD, IRRDR, and V3 of HCV genotype 1b cirrhosis patients being naive and nonresponders, treated with IFN (interferon) + ribavirin (RBN) by using an ultra-deep pyrosequencing method (UDPS).