Purpose of Investigation: We aimed to find a prevalence of human papilloma virus (HPV) in order to define the 100 genotypes and subset of 14 oncogenic genotypes in pregnant Turkish women and to compare these with non-pregnant women. Methods: Cervical thin-prep specimens were obtained from 164 women in the first trimester pregnancy and 153 non pregnant women. Results: 29.2% of pregnant versus 19.6% of non-pregnant Turkish women had at least one of the 100 types of HPV infection - a statistically significant difference. The rate of 14 high-risk HPV genotype infections was significantly higher in pregnant (14.6) compared to non-pregnant Turkish women (9.6%). Conclusions: Pregnant Turkish women are at higher risk for all HPV infections including high-risk cervical cancer genotypes.