Determination of Linezolid in Human Breast Milk by High-Performance Liquid Chromatography with Ultraviolet Detection

Sagirli O., Onal A., Toker S., Oztunc A.

JOURNAL OF AOAC INTERNATIONAL, vol.92, no.6, pp.1658-1662, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 92 Issue: 6
  • Publication Date: 2009
  • Journal Name: JOURNAL OF AOAC INTERNATIONAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1658-1662
  • Istanbul University Affiliated: Yes

Abstract

A rapid and simple HPLC method was developed for the determination of linezolid (LNZ) in human breast milk after a simple protein precipitation with methanol. The chromatographic separation was achieved on a C18 column (5 m, 250 4.6 mm id) using a mobile phase of acetonitrile10 mM acetic acid (25:75, v/v) at a flow rate of 1 mL/min. The LNZ peak was measured by photodiode array detection at 250 nm. The calibration graph was linear over the range of 0.520.0 g/mL. The limits of detection and quantitation were found to be 0.1 and 0.5 g/mL, respectively. The precision of the assay and the recovery of LNZ from breast milk at three different concentrations were assessed. The intraday and interday RSD values were found to be <5. The mean absolute recovery was 85.33. The developed method was successfully applied to the determination of LNZ in breast milk obtained from the breastfeeding mother after oral administration of LNZ.

A rapid and simple HPLC method was developed for the determination of linezolid (LNZ) in human breast milk after a simple protein precipitation with methanol. The chromatographic separation was achieved on a C18 column (5 mu m, 250 x 4.6 mm id) using a mobile phase of acetonitrile-10 mM acetic acid (25:75, v/v) at a flow rate of 1 mL/min. The LNZ peak was measured by photodiode array detection at 250 nm. The calibration graph was linear over the range of 0.5-20.0 mu g/mL. The limits of detection and quantitation were found to be 0.1 and 0.5 mu g/mL, respectively. The precision of the assay and the recovery of LNZ from breast milk at three different concentrations were assessed. The intraday and interday RSD values were found to be <5%. The mean absolute recovery was 85.33%. The developed method was successfully applied to the determination of LNZ in breast milk obtained from the breastfeeding mother after oral administration of LNZ.