In this work, we present a novel biometric authentication approach based on combination of AC/DCT features, MFCC features, and QRS beat information of the ECG signals. The proposed approach is tested on a subset of 30 subjects selected from the PTB database. This subset consists of 13 healthy and 17 non-healthy subjects who have two ECG records. The proposed biometric authentication approach achieves average frame recognition rate of %97.31 on the selected subset. Our experimental results imply that the frame recognition rate of the proposed authentication approach is better than that of ACDCT and MFCC based biometric authentication systems, individually.