Production of exopolysaccharide from probiotic Saccharomyces cerevisiae var. boulardii S11 and determination of its structural, rheological, antioxidant and prebiotic properties
Food Science and Biotechnology, cilt.35, sa.1, ss.141-151, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 35 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s10068-025-02018-3
- Dergi Adı: Food Science and Biotechnology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Biotechnology Research Abstracts, CAB Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
- Sayfa Sayıları: ss.141-151
- Anahtar Kelimeler: Antioxidant activity, Exopolysaccharide, Prebiotic activity, Rheological properties, S. boulardii
- İstanbul Üniversitesi Adresli: Evet
Özet
This study is the first to report the production, characterization, and technofunctional properties of exopolysaccharide (EPS) from the probiotic yeast S. boulardii S11 (Sb-EPS). HPLC analysis revealed that Sb-EPS has a heteropolysaccharide structure composed mainly of mannose and glucose. FT-IR and NMR analyses confirmed the polysaccharide structure of EPS. Rheological analysis showed that Sb-EPS exhibited non-Newtonian shear-thinning flow behavior. The EPS exhibited high antioxidant activity at a concentration of 4 mg/mL, with more than 50% radical scavenging capacity for DPPH and ABTS, and 117.72 ± 2.54 mg/mL for FRAP. Additionally, Sb-EPS demonstrated 90.11, 71.30, and 55.95% heavy metal adsorption capacities for Cu2⁺, Fe2⁺, and Zn2⁺, respectively. Furthermore, Sb-EPS’s ability to stimulate the growth of probiotic bacteria L. rhamnosus and L. plantarum to a greater extent than glucose and inulin suggests its potential as a prebiotic candidate. The findings indicate that Sb-EPS could be a promising, novel, and functional ingredient for food applications.