Antioxidant and Cytotoxic Activities of Aphanes arvensis Extracts

Hamad I., Erol-Dayi O., Pekmez M., Onay-Ucar E., Arda N.

PLANT FOODS FOR HUMAN NUTRITION, cilt.65, sa.1, ss.44-49, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/s11130-009-0151-y
  • Dergi Adı: PLANT FOODS FOR HUMAN NUTRITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.44-49
  • Anahtar Kelimeler: Antioxidant and cytotoxic properties, Methanol & water extracts, Salad plants, Antioxidant activity, Aphanes arvensis, Cytotoxicity, Reactive oxygen species, POSSIBLE INVOLVEMENT, POLYACRYLAMIDE GELS, SCAVENGING ACTIVITY, TOTAL PHENOLICS, PROTEINS, OXIDATION, CAPACITY, ASSAYS
  • İstanbul Üniversitesi Adresli: Evet

Özet

The objectives of this study were to examine the free radical scavenging activity and the protective effects against macromolecular oxidation as well as the cytotoxic activity of Aphanes arvensis aqueous and methanolic extracts. Free radical scavenging activity was determined by DPPH method. The methanolic extract showed a scavenging activity nearly equivalent to Trolox and Vitamin C and has an IC50 value of 4.54 A mu g/mL. Total antioxidant capacity was determined by CUPRAC method. The antioxidant capacity of aqueous and methanolic extract was 0.792 and 1.550 mmol TE/g DWE, respectively. The protective effect of A. arvensis extracts against lipid peroxidation was evaluated using a liposome oxidation system. The methanolic extract was more active than the aqueous extract. The aqueous extract possessed protective effect against protein oxidation in a dose dependent manner. Both extracts showed inhibitory effect on DNA oxidation as measured by plasmid relaxation assay. Results presented here indicate that A. arvensis possess strong antioxidant activity and protective effects with very little cytotoxic effect, and they can therefore be used as a natural additive in food, cosmetic and pharmaceutical industries.