Injectable and self-healing dual crosslinked gelatin/kappa-carrageenan methacryloyl hybrid hydrogels via host-guest supramolecular interaction for wound healing

Yilmaz-Aykut D., Torkay G., KAŞGÖZ A., Shin S. R., BAL ÖZTÜRK A., DELİGÖZ H.

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, vol.111, no.11, pp.1921-1937, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 111 Issue: 11
  • Publication Date: 2023
  • Doi Number: 10.1002/jbm.b.35295
  • Journal Name: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Computer & Applied Sciences, INSPEC, MEDLINE, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.1921-1937
  • Istanbul University Affiliated: No

Abstract

Injectable hydrogels based on natural polymers have shown great potential for various tissue engineering applications, such as wound healing. However, poor mechanical properties and weak self-healing ability are still major challenges. In this work, we introduce a host-guest (HG) supramolecular interaction between acrylate-beta-cyclodextrin (Ac-beta-CD) conjugated on methacrylated kappa-carrageenan (MA-kappa-CA) and aromatic residues on gelatin to provide self-healing characteristics. We synthesize an MA-kappa-CA to conjugate Ac-beta-CD and fabricate dual crosslinked hybrid hydrogels with gelatin to mimic the native extracellular matrix (ECM). The dual crosslinking occurs on the MA-kappa-CA backbone through the addition of KCl and photocrosslinking process, which enhances mechanical strength and stability. The hybrid hydrogels exhibit shear-thinning, self-healing, and injectable behavior, which apply easily under a minimally invasive manner and contribute to shear stress during the injection. Invitro studies indicate enhanced cell viability. Furthermore, scratch assays are performed to examine cell migration and cell-cell interaction. It is envisioned that the combination of self-healing and injectable dual crosslinked hybrid hydrogels with HG interactions display a promising and functional biomaterial platform for wound healing applications.